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Fig. 6 | Molecular Cancer

Fig. 6

From: Chemotherapeutic drugs stimulate the release and recycling of extracellular vesicles to assist cancer cells in developing an urgent chemoresistance

Fig. 6

Dynamin 2 and Clathrin mediate the endocytosis of ABCB1-containing EV in recipient cancer cells A KB cells are transfected with siRNAs targeted against caveolin-1 and control scramble sequence RNA (s.c.). B The decrease of caveolin-1 expression in KB cells has no significant effect on the intercellular transfer of ABCB1 in co-cultures. C-D Pretreatments of KB cells with caveolae pharmacological inhibitors, methyl-β-cyclodextrin (MβCD; 1 mM)(C) and filipin III (D), show no remarkable inhibitory effects on ABCB1 transfer when compared with the untreated control cells. E The expression of clathrin is decreased by shRNA targeting clathrin as compared with control shRNA in KB cells. The intercellular transfer rate of ABCB1 is decreased when the expression of clathrin is suppressed in KB cells. G Knockdown of dynamin2 using specific shRNA constructs is showed by immunoblot. H The suppression of dynamin-2 expression level in KB cells leads to a striking decrease in the intercellular transfer rate of ABCB1. I Pretreatment of KB cells with the inhibitor of dynamin GTPase Dynasore (10 μM) significantly reduces the rate of ABCB1 transfer, in the absence or presence of VCR. J Treatment with VCR did not alter the expression of clathrin, caveolin-1 and dynamin-2 in KB and KBv200 cells

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