Fig. 3
From: m6A mRNA methylation regulates CTNNB1 to promote the proliferation of hepatoblastoma
Silencing METTL3 inhibited HB tumor growth in nude mice models. a RT-qPCR was used to verify the METTL3 knockdown efficiency using shRNA in Huh6 cells. (independent-samples t-test). b Knockdown of METTL3 effectively suppressed the HB subcutaneous tumor growth in nude mice. c Tumor size and weight at the endpoint. Tumor size was monitored every 4th days. (two-way ANOVA followed by Bonferroni’s test and independent-samples t-test). d Western blotting was used to detect the expression of TCF4 and Cyclin D1 in METTL3 knockout HB cell Huh6. e Western blotting was used to detect the expression of TCF4 and Cyclin D1 in mice tumor-bearing tissue ns, no significant difference. *p-value< 0.05,**p-value< 0.01, ***p-value< 0.001, ****p-value< 0.0001