Fig. 1

LUCAT1 is transcriptionally induced by HIF-1α under hypoxia. a Candidate lncRNAs were identified by RNA-seq of hypoxia treated RKO cell, RNA-seq of CRC tissues and TCGA dataset. b Correlation between HIF-1α and each candidate lncRNAs in TCGA dataset. c Correlation between hypoxia signature genes and LUCAT1 was generated from GEPIA. n = 275 CRC tissues in COAD. pearson correlation. d RNAscope assay of LUCAT1 localization in HCT-116 cells without or with CoCl₂ treatment. e Relative LUCAT1 expression in HCT-116 and LoVo cells treated with CoCl₂ was determined by qPCR. n = 3 independent experiments, two-tailed Student’s t-test. f qPCR was performed to determine relative LUCAT1 expression in HCT-116 and LoVo cells transfected with siNC or HIF-1α siRNAs under hypoxia at serial time points. n = 3 independent experiments, two-tailed Student’s t-test. g Schematic diagram of HREs in LUCAT1 locus. h ChIP assay demonstrating the binding capacity of HIF-1α to each HRE were conducted in HCT-116 and LoVo cells treated with hypoxia for 24 h. i Luciferase reporter assay for the second HRE activity of LUCAT1 in HCT-116 and LoVo cells with the indicated treatment. n = 5 independent experiments, two-tailed Student’s t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001