Fig. 5

PTBP1 functions downstream of LUCAT1 in CRC under hypoxia. a Cell cycle (b) Western blotting for p-H2AX and (c) Caspase 3/7 activity assays in CRC cells transfected with siNC or PTBP1 siRNAs. n = 3 independent experiments, two-tailed Student’s t-test. Integrated Density Value (IDV) was obtained by ImageJ and normalized by the first lane. d CCK8 assays in LUCAT1-depleted CRC cells following reintroduction of PTBP1. n = 3 independent experiments, two-tailed Student’s t-test. e Tumor volume and tumor weight of indicated xenograft mouse model. n = 6 tumors, two-tailed Student’s t-test. f Western blotting for p-H2AX expression in LUCAT1-depleted CRC cells following reintroduction of PTBP1. Integrated Density Value (IDV) was obtained by ImageJ and normalized by the first lane. g qPCR assays of alternative splicing events in LUCAT1-depleted CRC cells following reintroduction of PTBP1. n = 3 independent experiments, two-tailed Student’s t-test. h RIP assays were performed in HCT-116 and RKO cells to evaluating the binding capacities between PTBP1 and target genes under normoxia or hypoxia. n = 3 independent experiments, two-tailed Student’s t-test. i Alternative splicing events in each target gene were determined by qPCR and normalized to siNC-transfected CRC cells under normoxia. n = 3 independent experiments, two-tailed Student’s t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001