Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: m6A demethylase ALKBH5 inhibits pancreatic cancer tumorigenesis by decreasing WIF-1 RNA methylation and mediating Wnt signaling

Fig. 5

WIF-1 as a downstream target of ALKBH5-mediated m6A modification. a Megagene profiles depicting global changes in m6A binding surrounding 5′ and 3′ end UTRs in BxPC-3 and MIA Paca-2 cells transfected with control and/or ALKBH5 vector. Right panel is a list of top 10 genes that most significantly changed in m6A modification in either 5′ or 3′ end UTRs. b Tracks of m6A sequence of WIF-1 mRNA in cells described in (a). (c) Venn diagram showing the overlap of genes differentially expressed in AsPC-1 and PANC-1 cells transfected with shALKBH5. d Expression of WIF-1 mRNA in BxPC-3 cells transfected with control and/or ALKBH5 vector determined by qRT-PCR. Enrichment of a Wnt signaling gene expression signature in transformed (e) AsPC-1 (f) MIA Paca-2 cells described above

Back to article page

Molecular Cancer

ISSN: 1476-4598