Fig. 8

WIF-1 is critical to ALKBH5 suppressed Wnt signaling. a Immunoblotting of lysates from BxPC-3 cells transfected with control, ALKBH5, shCtrl, and/or shWIF-1. Expression of ALKBH5 and WIF-1 were measured. β-actin was used as a loading control. b MTT, c Colony formation, d Migration, e Invasion, and (f) Immunoblotting of WIF-1 downstream targets in cells described in (a). (g) Immunoblotting of lysates from AsPC-1 cells transfected with shCtrl, shALKBH5, control, and/or WIF-1 vector. Expression of ALKBH5 and WIF-1 were measured. β-actin was used as a loading control. (h) MTT, (i) Colony formation, (j) Migration (k) Invasion, and (l) Immunoblotting of WIF-1 downstream targets in cells described in (g). m Representative ventral view images and its quantification (n) of bioluminescence from xenograft mice implanted with AsPC-1 cells described in (g). o Quantification of IHC staining for Ki67 in tumor sections from xenograft mice described above. Scale bars = 50 μm (d, e, j and k). The data are shown as the means ± S.D. **P <0.01; ## <0.01