Fig. 3
From: circRIP2 accelerates bladder cancer progression via miR-1305/Tgf-β2/smad3 pathway
Over-expressing circRIP2 promotes bladder cancer progression. Bladder cancer cells that stably over-expressed circRIP2 was constructed. a Level of circRIP2 in 5637 and U3 cells after circRIP2 overexpression were detected by qPCR. Vector was taken as normal control; b, c. CCK8 assay was performed to detect cell viability of bladder cancer cells; d, e. Cell potential to replicate and self-renew were reflected by clone formation assay; f, g, h. Rate of wound healing assay showed cell potential of migration; scale bar: 100 μm; i, j, k, l Trans-well migration and matrigel invasion assay showed cell potential of migration and invasion; scale bar: 25 μm; m, n, o Over-expressed circRIP2 bladder cancer cells were injected subcutaneously to detect in vivo effect of circRIP2. n = 5 mice for each group.