Fig. 3
From: Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells
P14AS affects the expression level of the ANRIL gene. qRT-PCR data are normalized to GAPDH mRNA levels and shown as the means ± SD. a The expression levels of ANRIL in the P14AS vector stably transfected colon cancer cells (HCT116 and SW480), and gastric cancer cells (MGC803) were analyzed by qRT-PCR. b GFP-expression efficiency in the P14AS expression and control vector in the stably transfected cells. c Knockout (KO) of the ARE-containing element within P14AS exon 1 by CRISPR/Cas9. A 416-bp (chr9: 21,989,309-21,989,724) or 514-bp (chr9: 21,989,211-21,989,724) fragment deletion in P14AS exon 1 was detected by RT-PCR (bottom chart) in HEK293T or HCT116 P14AS-KO clones. d The expression change of ANRIL in HCT116 and HEK293T cells whose ARE-containing elements in P14AS exon 1 were homogenously deleted in the qRT-PCR analysis. Pooled P14AS ARE-KO-negative clones were used as the wild-type (WT) control