Fig. 5
From: Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance
Decreased tumor burden in mouse model injected subcutaneously with MUC5AC knockdown cell line. a Schematic representation of subcutaneous injection of HCT-8 scrambled (Scr) and MUC5AC knockdown (Sh5AC) cells in athymic nude mice. b and c Mice injected with Sh5AC cell lines exhibited decreased tumor volume and weight as compared to Scr animals. d Immunoblotting of various signaling molecules associated with MUC5AC-CD44 interaction in subcutaneous tumor tissue lysate. e Expression of transmembrane mucin MUC4 was lower in colon cancer patients compared with healthy controls. f Correlation between MUC4 and MUC5AC mRNA expression in colon cancer patients (TCGA-COAD dataset). g and h In vitro knockdown and knockout of MUC5AC showed an increase in MUC4 expression (analyzed by anti-MUC4 8G7 antibody). Tumor tissues from mice subcutaneously injected with Sh5AC cells also expressed higher MUC4 as compared to Scr group