Fig. 6
From: Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance
MUC5AC expression increases tumor burden in orthotopic mouse model. a Schematic representation of colon orthotopic mouse model. Approximately 2 × 106 cells (HCT-8 parental and MUC5AC knockout, labelled with luciferase) were injected in the serosa of the caecal region. b and c In vivo tumor growth was imaged using the IVIS imaging system after intraperitoneal injection of D-luciferin (150 mg/kg) at days 20 and 50. Photon counts from both parental and KO clones were quantified 20 and 50 days post-implantation. d Representative hematoxylin and eosin stain of parental and KO cells injected orthotopically in athymic nude mice. Higher tumor burden was observed in the colon of mice injected with parental cells