Fig. 2
From: CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer
Ferroptosis-related exo-miR-522 mainly derived from CAFs. a. Immunofluorescence staining for α-SMA, FAP, and FSP1 expression of NFs and CAFs (scale bar, 20 μm). b. Western blot analysis of α-SMA, FAP, and FSP1 protein levels in six paired NFs and CAFs (n = 6). c. Comparison of miR-522 levels in primary NFs, tumor cells (TCs) and CAFs (n = 12). d. TEM image of exosomes isolated from primary NFs, TCs and CAFs (scale bar, 100 nm). e. Western blotting analysis of CD63, Alix, and Tsg101 in exosomes. f. Relative levels of miR-522 in the exosomes described above (n = 12). g. The levels of exo-miR-522 derived from CAFs is negatively linked with ALOX15 expression (n = 12). h. CAF exo-miR-522 is negatively related with lipid-ROS levels (n = 12). ** indicates p < 0.01 and *** indicates p < 0.001