Fig. 5

EZH2 loss suppresses migration and invasion of UM cells. a GSK126 inhibited the wound healing ability in UM cells. 92.1 and Mel270 cells were pre-treated with 15.0 μmol/L of GSK126, and then subjected to wound healing assay. Photos were taken in the same field at the indicating time. Original magnification, 100× (scale bar, 100 μm), Olympus IX71. b-c Depletion of EZH2 suppressed the migration of UM cells. 92.1 and Mel270 cells transfected with Scramble, or lentiviral shEZH2 in the absence or presence of EZH2-encoding constructs were subjected to transwell assay (b, left); the number of migrated cells was normalized relative to scramble (b, right). Data of bar charts were from 3 random fields. ***, P < 0.0001, one-way ANOVA, post hoc intergroup comparisons. After pre-treated with 15.0 μmol/L GSK126 for 24 h, 92.1 and Mel270 cells were evaluated for migration ability by transwell assay (c). Data of bar charts were from 3 random fields. ***, P < 0.0001, Student’s t test. d-f Depletion of EZH2 suppressed the invasion of UM cells. 92.1 and Mel270 cells transfected with Scramble, or lentiviral shEZH2 in the absence or presence of EZH2-encoding constructs were subjected to transwell invasion assay (d, left); the number of the invaded cells was normalized relative to scramble (d, right). Data of bar charts were from 3 random fields. ***, P < 0.0001, one-way ANOVA, post hoc intergroup comparisons. After treated with 15.0 μmol/L GSK126 for 24 h, 92.1 and Mel270 cells were seeded for transwell invasion assay (e). Data of bar charts were from 3 random fields. **, P < 0.01; ***, P < 0.0001, Student’s t test. 92.1 and Mel270 cells were treated with GSK126 as indicated for 24 h, followed by Western blotting analysis for MMP2 and MMP9 (f)