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Fig. 5 | Molecular Cancer

Fig. 5

From: Dying tumor cell-derived exosomal miR-194-5p potentiates survival and repopulation of tumor repopulating cells upon radiotherapy in pancreatic cancer

Fig. 5

Dying tumor cell-released PGE2 promotes proliferation of the recovered TRCs. a ELISA detection of PGE2 contents in the supernatant of 10Gy irradiated or unirradiated SW1990 cells. b qPCR quantification of PTGS2 expression in SW1990 cells before and after 10Gy radiation. c Protein level of COX-2, RAB27A, TSG101 and CD9 in SW1990 cells before and after 10Gy radiation. d IHC staining of CD63, CD9 and COX-2 in PDX tumor tissue before and after 10Gy radiation. Scale bar: 50 μm. e Bioluminescence intensity of reporter cells that were cultured alone or co-cultured with feeder cells and treated with or without celecoxib as indicated. f Cell viability assay of PANC-1 cells treated with different concentration of PGE2 immediate after 10Gy/0Gy radiation. g Western blot detecting the expression of COX-2, RAB27A, TSG101 and CD9 in SW1990 cells treated with 10Gy radiation and different concentration of celecoxib. Data are presented as mean with SD of at least three independent experiments; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS, not significant from unpaired Student’s t test

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Molecular Cancer

ISSN: 1476-4598