Fig. 4

circ_0020710 upregulates the CXCL12 level via sponging miR-370-3p. a The heatmap of differentially expressed mRNAs in A2058 cells transfected with Vector or circ_0020710. Each sample was mixed with three replicates. b Volcano plot of differentially expressed mRNAs in A2058 cells transfected with Vector or circ_0020710. c and d GO and KEGG analyses of differentially expressed mRNAs in A2058 cells. e Schematic illustration exhibiting overlapping of miR-370-3p target mRNAs and upregulated DEGs. f Putative binding site of miR-370-3p with respect to CXCL12 was predicated via StarBase v3.0. g The luciferase activity of pLG3-CXCL12 in HEK-293 T cells after co-transfection with miR-370-3p. h and i Relative CXCL12 mRNA and protein expression in melanoma cells with different treatments. Unpaired student’s t-test and one-way ANOVA test were used for the statistical analyses. **p < 0.01; ***p < 0.001;****p < 0.0001; ns, no significant