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Fig. 2 | Molecular Cancer

Fig. 2

From: Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2′-O-methylation via NOP58 recruitment in colorectal cancer

Fig. 2

The expression of ZFAS1, SNORD12C, and SNORD78 and subcellular localization. a and b, Expression levels of ZFAS1, NOP58 (a) and SNORD12C or SNORD78 (b) in CRC cell lines including HT29, HCT116, SW480, and SW620 and normal intestinal epithelial cell line HIEC detected by RT-PCR and qPCR assay. Values are the mean ± s.d. of n = 3 independent experiments. c, Representative images of ZFAS1, NOP58, SNORD12C and SNORD78 expression in paired CRC and matched adjacent normal tissues determined by RT-PCR assays (5 representative data was shown). d, Co-localization of ZFAS1, SNORD12C and SNORD78 with NOP58 protein detected by ISH and IF assays in HCT116 cells. Scale bar = 5 μm. e and f, The expression of ZFAS1, NOP58, SNORD12C, and SNORD78 after overexpression or silencing ZFAS1 in both of HCT116 and SW620 cells by qRT-PCR assay.*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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