Fig. 6

circCCDC9 serves as a miRNA sponge of miR-6792-3p to regulate CAV1 expression. a and b. Relative mRNA and protein levels of CAV1 were detected in MKN-45 cells transfected with NC, si-circ and inhibitor and AGS cells transfected with NC, oe-circ and mimics using qRT-PCR and western blot, respectively. c. FISH was performed to observe the cellular location of circCCDC9 (red) and miR-6792-3p (green) in cells (magnification, 400×, scale bar, 100 μm). d. Schematic illustration of circCCDC9-WT and circCCDC9-MUT luciferase reporter vectors. e. The relative luciferase activities were detected in 293 T cells after co-transfection with circCCDC9-WT or circCCDC9-MUT and mimics, inhibitor or NC, respectively. f and g. Anti-Ago2 RIP assay was executed in MKN-45 cells after transfection with mimics and NC, followed by nucleic acid electrophoresis and qRT-PCR to detect circCCDC9 and miR-6792-3p, respectively. h and i. RNA pull-down assay was executed in MKN-45 cells, followed by qRT-PCR to detect the enrichment of circCCDC9 and miR-6792-3p. j. Pearson correlation analysis of the correlation of circCCDC9 with miR-6792-3p expression based on GC tissues. Data were showed as mean ± SD; ns indicated no significance. **P < 0.01, ***P < 0.001