Fig. 2

LncRNA BCRT1 knockdown inhibited breast cancer cell proliferation in vitro and in vivo. a The expression levels of lncRNA BCRT1 in MDA-MB-231 and MDA-MB-468 cells after transfection with si-NC or si-BCRT1 were detected by RT-PCR. b-c The effects of lncRNA BCRT1 knockdown on the proliferation of MDA-MB-231 and MDA-MB-468 cells were examined by MTT assay (b) and colony formation assays (c). Experiments were performed in triplicate. d EdU assays were used to detect the proliferation rate of MDA-MB-231 and MDA-MB-468 cells after lncRNA BCRT1 knockdown. Columns are the average of three independent experiments. e Flow cytometry was performed to determine the effect of lncRNA BCRT1 on apoptosis by flow cytometry analysis. f RT-PCR was used to determine the efficiency of the lncRNA BCRT1-overexpressing vector. g MTT assay indicated an increased proliferative ability of MDA-MB-231 and MDA-MB-468 cells after lncRNA BCRT1 overexpression. h MDA-MB-231 cells were stably transfected with the lncRNA BCRT1-overexpressing vector or control vector and injected subcutaneously into nude mice. Compared with the vector group, lncRNA BCRT1 overexpression promoted tumor growth. i Tumor volume and weight were significantly increased in the lncRNA BCRT1-overexpressing group. j Representative images of H&E and Ki67 staining in the tumor. Immunohistochemical staining revealed that lncRNA BCRT1 overexpression led to increased expression of Ki67. (**P < 0.01 and ***P < 0.001)