Fig. 3

Establishments of murine and human HCC cells with EMT-like features and immunosuppression by circMET a. Volcano plots show the differentially expressed genes in HCC cells with different circMET expression levels; b. GO and KEGG analyses were performed on the differentially expressed genes; c. The cellular morphology of HCC cells with silenced or forced expression of circMET; d. The expression of MET was determined by qRT-PCR in HCC cells with silenced or forced expression of circMET; e. The expression of E-cadherin, vimentin, FN1 and Snail was determined by qRT-PCR in HCC cells with silenced or forced expression of circMET; f. The levels of E-cadherin, vimentin, FN1 and Snail protein were determined by western blotting in HCC cells with silenced or forced expression of circMET; g. circMET levels were elevated in Hep1–6 cells transfected with a circMET expression plasmid; h. Tumorigenesis of Hep1–6-control and Hep1–6-circMET cells in C57BL/6 mice, and the tumor burden of Hep1–6-circMET cells was larger than that of Hep1–6-control cells; i. Chemokine chips were used to determine the differences in chemokines between the sera of mice implanted with Hep1–6-control and Hep1–6-circMET cells; j. The top ten different chemokines between the sera of mice implanted with Hep1–6-control and Hep1–6-circMET cells were further assayed by ELISA; k. CD4⁺, CD8⁺ and Treg T cells were evaluated by immunohistochemistry in tumors derived from Hep1–6-control and Hep1–6-circMET cells; l. The histogram shows the CD4⁺, CD8⁺ and Treg T cells in tumors derived from Hep1–6-control cells and Hep1–6-circMET cells