Fig. 3

Silencing LINC00173.v1 represses proliferation and migration of vascular endothelial cells. a Effect of LINC00173.v1 expression in lung cancer cells on human umbilical vein endothelial cells (HUVECs) in tube formation assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test or one-way ANOVA test. Scale bars, 200 μm. b Effect of LINC00173.v1 expression in lung cancer cells on migration ability of HUVECs in transwell assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test or one-way ANOVA test. Scale bars, 200 μm. c Effect of LINC00173.v1 on length of the 2nd and 3rd vessels on chick embryo chorioallantoic membrane (CAM). Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test or one-way ANOVA test. d Real-time PCR analysis of the effect of LINC00173.v1 on expression of multiple proliferation and migration of vascular endothelial cells-associated (angiogenesis-associated) genes. Pseudo-color scale values were log2 transformed. Transcript levels were normalized by GAPDH expression. The experiment was independently performed three times. e Effect of LINC00173.v1 on secretion level of VEGF-A in lung cancer cells by enzyme linked immunosorbent assay (ELISA). Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test or one-way ANOVA test