Fig. 4

Silencing LINC00173.v1 inhibits tumorigenesis in lung cancer cells. a Images of the representative excised tumors from the mice at 35 days after injection with the indicated cells. b Average weight of excised tumors from the indicated mice (n = 6). Each bar represents the median values ± quartile values. P value was determined by unpaired t test. c Tumor volumes (n = 6) were measured every 7 days. Each bar represents the median values ± quartile values. *P < 0.05 by unpaired t test in final measurement. d and e Representative sections and CD31⁺ lymphatic or blood vessel (LBV) density in tumor tissues from the indicated mice groups (n = 6). after 5 weeks of cell injection. Each bar represents the median values ± quartile values. P value was determined by unpaired t test. Scale bars of 100× magnification, 200 μm and 400× magnification, 50 μm. f Metastatic lung tumor nests (red arrow) in the indicated mice group. Lung metastatic tumor tissues in mice were confirmed by H&E staining. Scale bars, 200 μm. g and h Tumor cell number per mm² and tumor necrotic area in lung H&E section from the indicated mice groups after 5 weeks of tail veins injection. Each bar represents the median values ± quartile values. P value was determined by unpaired t test. i and j Kaplan–Meier analysis of the effect of LINC00173.v1 overexpression (i) and downexpression (j) on cumulative survival in the indicated mice groups (n = 8). P value was determined by Log-rank test