Fig. 2

KDM5C-mediated demethylation of H3K4me3 inhibited METTL14 transcription. a. Analysis of H3K4me3 ChIP-Seq data of CRC cells and colorectal tissues in the METTL14 locus. b. The mRNA levels of METTL14 in KDM5A-IN-1(5 μM)-treated HCT116 and HCT8 cells were measured using qRT-PCR. c. The METTL14 and H3K4me3 protein levels in HCT116 and HCT8 cells were detected by western blot after KDM5A-IN-1(5 μM) treatment. d. The KDM5C knockdown efficiency was determined at the protein levels in HCT116 and HCT8 cells using western blot. e. The METTL14 and H3K4me3 protein levels in HCT116 and HCT8 cells were detected using western blot. f. ChIP assays were employed to measure the levels of KDM5C binding f and the enrichment of H3K4me3 g at the promoter of METTL14 in KDM5C deficiency or control HCT116 and HCT8 cells. ***P < 0.001