Fig. 4

circTLK1 acts as a sponge for miR-136-5p in RCC cells. a and b circTLK1 is mainly located in the cytoplasm of ACHN. c circTLK1 in ACHN cells was pulled down by a circTLK1-specific probe and detected by a qRT-PCR assay. d miR-136-5p was pulled down by using a circTLK1-specific probe. e Schematic illustration of circTLK1-WT and circTLK1-Mut luciferase reporter vectors. f Relative luciferase activities were investigated in RCC cells after transfection with circTLK1-WT or circTLK1-Mut and miR-136-5p mimic or miR-NC. g and h The expression level of miR-136-5p was evaluated by performing a qRT-PCR assay in RCC cells transfected with circTLK1 vector or shcircTLK1. i and j circTLK1 expression in RCC cells transfected with a miR-136-5p mimic or a miR-136-5p inhibitor. *p < 0.05, **p < 0.01, no significant (NS)