Fig. 6

RBAT1 interacts with HNRNPL and E2F3 promoter region. a Technical description of the ChIRP method. b ChIRP-MS revealed that sixteen proteins bound to RBAT1 in Y79, and eight proteins bound to RBAT1 in 5637. HNRNPL was the only overlapping protein in Rb and BCa cell lines. c The ChIRP-MS results were verified by western blotting. RBAT1 oligos indicate the biotinylated antisense oligonucleotides against RBAT1. Ctrl oligos indicate the scrambled oligonucleotides, and THRIL oligos were selected as a positive control. d The interaction of RBAT1 with HNRNPL was verified by RNA immunoprecipitation (RIP) assays. The results are shown as the mean ± SD in three independent experiments. **p < 0.01. A validated HNRNPL interacting LncRNA THRIL served as a positive control. IgG antibody and U6 RNA served as negative controls. e-g ChIRP-PCR revealed that RBAT1 bound specifically to the E2F3 promoter. The GAPDH promoter region was selected as the negative control. NC: untreated cell lines; and Ctrl: cell lines transfected with GapmeR control. The results are shown as the mean ± SD in three independent experiments. NS means no significant difference, ** p < 0.01