Fig. 2

circPACRGL is significantly upregulated in CRC cells with tumor-derived exosomes addition. a Heat maps of the expression profile consisting of three pairs of exosome-stimulated HCT116 and SW480 cells (Ex-HCT116 and Ex-SW480), and control cells by RNA sequencing. b A Venn diagram of the intersection between results of the differential expression of HCT116 and SW480 cells. c, d and e qRT-PCR analyses of circPACRGL mRNA in HCT116 and SW480 after CRC-derived exosomes (c), CRC patients’ serum-derived exosomes (Serum-EXO) (d), or tumor-derived exosomes (Tumor-EXO) (e) treatment, respectively. f and g qRT-PCR analyses of circPACRGL mRNA in HCT116 and SW480 cells after transfected with si-NC or si-circPACRGL either with or without tumor-derived exosomes (Tumor-EXO) addition. All P values were determined by a two-tailed unpaired student’s t-test (**, P < 0.01); si, siRNA; NC, Negative Control. Ex, exosomes