Fig. 4

TGF-β1 is a common target of miR-142-3p/miR-506-3p. a Schematic representation of the 3′-UTR of TGF-β1 with the predicted target site for miR-506-3p/miR-142-3p. The mutant site of TGF-β1 3′-UTR is indicated (without lines). b Luciferase reporter analysis was performed to examine the binging ability between miR-506-3p/miR-142-3p and TGF-β1. Reporter constructs containing either TGF-β1 wt or TGF-β1 mut at the predicted miR-142-3p/miR-506-3p target sequences were co-transfected into HEK293T cells, along with miR-142-3p/miR-506-3p or miR-NC. c and d Western blots and qRT-PCR analyses of TGF-β1 expression in HCT116 and SW480 cells after transfected with miR-142-3p/miR-506-3p mimics or miR-NC. e and f Western blots and qRT-PCR analyses of TGF-β1 expression in HCT116 and SW480 cells after treatment with CRC-derived exosomes, along with miR-142-3p/miR-506-3p mimics or miR-NC. GAPDH was used as a loading control. All P values were determined by a two-tailed unpaired student’s t-test (**, P < 0.01, compared to the control group; #, P < 0.1, ##, P < 0.01, compared to the Ex + miR-NC group). Ex, exosomes