Fig. 1

WNT/β-catenin signaling activates HSF1. a Chemicals influencing gene expression in a similar manner to HSF1 inhibition were screened by connective map analysis. b The correlation of WNT/β-catenin signaling signature and HSF1 signature was detected by GEPIA. c The effect of Pyrvinium on HSE-driven promoter activity was explored by luciferase reporter assay. d The effects of Pyrvinium on the targets of HSF1 were analyzed by RT-PCR. e Binding of HSF1 to the promoters of HSF1 targets in CRC cells treated with or without Pyrvinium was determined by ChIP. f The luciferase assays of HSE before and after β-catenin knockdown were shown as in C. g and h The mRNA levels of HSF1 targets with β-catenin knockdown or LiCl treatment were analyzed by RT-PCR. i Binding of HSF1 to its targets promoter in CRC cells before and after β-catenin knockdown was analyzed by ChIP. j Volcano plot displays differentially regulated genes in dHSF1 compared to WT parental cells with LiCl. Red dots indicate significantly regulated genes based on adjusted p-value and log-fold change (logFC) (p < 0.01, log2FC > 2). k Differential gene expression analysis in WT and HSF1+/− MEF treated with LiCl were performed by RNA-seq. Numbers of upregulated genes in two cells were shown in Venn graph. l The correlation of 875 putative HSF1-dependent genes from K with reported HSF1 signature was detected by GEPIA. m Numbers of 875 putative HSF1-dependent genes with or without HSE in their promoters were summarized. n Representative HSF1 ChIP-seq tracks (NCBI GEO: GSE57398) for 368 HSE-containing genes are shown. Asterisks (*) indicate p < 0.05