Fig. 3

HSF1 protein translation is regulated by miR455-3p. a Overlap of HSF1-targeting microRNAs predicted by TargetScan, miRDB and StarBase. b The effect of miR455-3p on HSF1 protein was analyzed by western blotting. c The effect of miR455-3p inhibitor on β-catenin knockdown-induced HSF1 downregulation was determined by western blotting. d Luciferase activity assay was used to analyze the effect of miR455-3p on the activity of 3′-UTR with or without miR455-3p binding sites (*p < 0.05). e The binding between biotin-miR455-3p and HSF1 mRNA was determined by biotin pull down assay (*p < 0.05). f Expression of miR455-3p in 12 pairs of fresh CRC tissues and adjacent non-tumor tissues was analyzed by qPCR. g The correlation of HSF1 protein and miR455-3p in 12 pairs of fresh CRC tissues and adjacent non-tumor tissues was analyzed. h The effect of miR455-3p on viability of CRC cells was explored by MTS assay. i The effect of miR455-3p on apoptosis of CRC cells was analyzed using flow cytometry after PI and annexin V-FITC double staining. j Apoptosis of CRC cells treated with or without miR455-3p was determined by western blotting