Fig. 2

Tumor suppressor functions of miPEP133 in nasopharyngeal carcinoma (NPC) cells. a RT-QPCR of miPEP133 mRNA in the nuclear and cytoplasmic fractions of NP69 cells. U6 and GAPDH were used as control genes. b Representative images of miPEP133 western blot in NP69 and NPC cell lines. c Quantification of miPEP133 band intensity in western blot. Student’s t-test, **p < 0.005. d RT-QPCR of miPEP133 in normal pharynx and NPC samples (n = 8). Student’s t-test, *p < 0.05. e Western blot of miPEP133 in three NPC cell lines transfected with control (−) or miPEP133 plasmid (+). f Quantification of miPEP133 band intensity in western blot. Student’s t-test, **p < 0.005. g Cell growth rates of NPC cell lines. Two-way ANOVA followed by Sidak’s test, *p < 0.05, **p < 0.005, ***p < 0.001, #p < 0.0001. h Flow cytometry dot plots of C666–1 cells stained with AnnexinV/7-ADD. i Summarized flow cytometry results of AnnexinV/7-ADD-staining in NPC cells lines. Student’s t-test, *p < 0.05. j Cell cycle status of C666–1 cells determined by PI staining and flow cytometry. k Summarized flow cytometry results of PI-staining in three NPC cells lines. Student’s t-test, *p < 0.05. l Representative images of wound healing assay at 0 h and 24 h of control C666–1 cells and the miPEP133-overexpressing C666–1 cells. m Quantification of wound closure of control C666–1 cells and the miPEP133-overexpressing C666–1 cells at 24 h. Wound closure was presented as the percentage of wound width that was closed. Student’s t-test, #p < 0.0001. n Representative images of migrating cells in trans-well assay. o Quantification of migrating cells in the transwell migration assay. Student’s t-test, #p < 0.0001. p Representative images of invading cells in trans-well assay. q Quantification of invading cells in the transwell invasion assay. Student’s t-test, #p < 0.0001