Fig. 3
From: Identification of miPEP133 as a novel tumor-suppressor microprotein encoded by miR-34a pri-miRNA
Tumor suppressor functions of miPEP133 in NPC in vivo. a Tumor growth curves of nude mice that were injected with control C666–1 cells or miPEP133-overexpressing C666–1 cells. Two-way ANOVA test followed by Sidak test, *p < 0.05, **p < 0.005, ***p < 0.0005. b Tumors xenografts from nude mice. c Western blot images of miPEP133 in the tumors formed by control C666–1 cells and miPEP133-overexpressing C666–1 cells. d QPCR of miPEP133 in tumors. Student’s t-test, p < 0.01. e QPCR of miR-34a in the tumors. Student’s t-test, p < 0.01. f Representative images of Ki67, TUNEL, and cleaved caspase-3 staining in tumors from nude mice. DAPI was used to stain the nuclei. g Quantification of Ki67 positive proliferating cells in mouse tumors. Student’s t-test, ***p < 0.001. h Quantification of TUNEL positive apoptotic cells in mouse tumors. Student’s t-test, #p < 0.0001. i Quantification of cleaved capsase-3 positive apoptotic cells in mouse tumors. Student’s t-test, #p < 0.0001. j Decreased mRNA levels of miPEP133 were associated with advanced NPC in 85 human tumor samples. Student’s t-test, *p < 0.05, **p < 0.005. k mRNA levels of miPEP133 were lower in recurrent NPC. Student’s t-test, **p < 0.005. l mRNA levels of miPEP133 were lower in metastatic NPC. Student’s t-test, *p < 0.05. m High level of miPEP133 mRNA is a favorable prognostic marker for NPC patient. Kaplan–Meier analysis and log-rank test were performed (p = 0.014)