Fig. 6

hsa_circ_0004872 serves as a sponge for miR-224 to inhibit the tumor growth and metastasis of GC in vivo. a The nude mice were subcutaneously injected with BGC-823 cells stably transfected with hsa_circ_0004872 (pLCDH-circ_0004872) (right) or the control BGC-823 cells (pLCDH-circ) (left) in the two flanks of the mice.After 6 days, the subcutaneous tumor size was measured every 3 days and the tumor volume was calculated. b The tumor-bearing mice and the dissected tumors were photographed and shown. A ruler was used to indicate the size of the tumors. c The tumor weight with different cells was shown. d Hematoxylin and eosin (HE) staining was used to detect the tumors. e qRT-PCR analysis of the expression of hsa_circ_0004872 in the tumors. f qRT-PCR analysis of the expression of miR-224 in the tumors. Data were the means ± SD. g IHC analysis of the expression of p21 and Smad4 in the tumors. h Statistical analysis of the percentage of IHC positive exprssion cells in the tumors. i BGC-823 cells stably transfected with hsa_circ_0004872 (pLCDH-circ_0004872) or the control BGC-823 cells (pLCDH-circ) were injected into the tail vein of the nude mice. Six weeks later, the nude mice were dissected. The mice lungs were weighed. j Hematoxylin and eosin (HE) staining of lung tissues was used to detect the metastasis nodules. k The metastatic nodules in the mice lung were counted. All datas were the means ± SD