Fig. 6

JPX promoted lung tumor growth and metastasis in vivo by regulating miR-33a-5p. Rescue effects of JPX overexpression on AgomiR-33a-5p-mediated inhibition of tumor growth in mice subcutaneously implanted inhibition with SPP-A1 and NCI-H1299 cells. (a) Representative subcutaneous xenograft tumors were shown (n = 3). (b) Tumor volume and weight were measured. (c) Representative images of gross lung tissue lesions and (d) H&E-stained sections from the nude mice with tail vein injection in the rescue experiments. (e) Representative IHC staining of Twist1 and β-catenin in the tumors from nude mice with subcutaneous implantation in the rescue experiments. Scale bar = 100 μm. Data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001