Fig. 1

Circ_001680 is overexpressed in colorectal cancer. a Circ_001680 is generated from the B2M gene, located on chromosome 15. b Upper: Sequencing results showing the back-splice junction sequences of circ_001680. Lower: circ_001680 sequences that were PCR-amplified from cDNA and gDNA with divergent primers, respectively. c Relative expression levels of circ_001680 in 42 paired human colorectal cancer tissues. d The Mann-Whitney test was used to detect the relative expression level of circ_001680 in 42 normal and noncancerous tissues(***P < 0.005). e Correlation between circ_001680 expression and the stage of clinical T classification in 42 cases of CRC and noncancerous tissues (**p < 0.01, *p < 0.05). f Graphical illustration of the statistical circ_001680 distribution in CRC patients. (**p < 0.01, *p < 0.05). g qRT-PCR analysis of circ_001680 expression in 11 CRC cell lines