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Fig. 1 | Molecular Cancer

Fig. 1

From: RETRACTED ARTICLE: Exosome-mediated lncRNA AFAP1-AS1 promotes trastuzumab resistance through binding with AUF1 and activating ERBB2 translation

Fig. 1

LncRNA AFAP1-AS1 is upregulated in trastuzumab-resistant cells and activated by H3K27ac. a Volcano plot images were drawn to show the differentially expressed genes between SKBR-3 and SKBR-3-TR cells. Five samples were used in each group. b The top 20 dysregulated lncRNAs were shown in heat map between SKBR-3 and SKBR-3-TR cells. c qRT-PCR was performed to measure the differential expression of AFAP1-AS1 in trastuzumab-resistant cells and parental sensitive cells, **P < 0.01. d qRT-PCR showed that AFAP1-AS1 was upregulated in HER-2 positive patients compared to HER-2 negative patients. e The H3K27ac enriched area at the promoter of AFAP1-AS1 was predicted online at http://genome.ucsc.edu (shown as purple area). f RIP assay using anti-H3K27ac antibody showed that H3K27ac was enriched at the promoter region of AFAP1-AS1. Moreover, the enriched level was significantly increased in trastuzumab-resistant cells in contrast to parental sensitive cells, **P < 0.01. g RIP assay showed that trastuzumab treatment increased the enrichment of H3K27ac at AFAP1-AS1 promoter in SKBR-3 and BT474 cells, *P < 0.05, **P < 0.01. h The acetyltransferase inhibitor, C646, significantly suppressed the binding level of H3K27ac, *P < 0.05. i C646 treatment significantly inhibited the expression of AFAP1-AS1 in SKBR-3-TR and BT474-TR cells, * P < 0.05

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Molecular Cancer

ISSN: 1476-4598