Fig. 4

METTL14 suppressed the growth and invasion of CRC by targeting lncRNA XIST. a Volcano plot showed DEGs between shMETTL14 and control cells. METTL14 and interested genes which were reported to possess multiple m6A residues were indicated with black circles. b The levels of XIST were examined by real-time PCR in HCT116 and HT29 cells transfected with siNC or siMETTL14, respectively. **, p < 0.01. c Real-time PCR showing expression levels of XIST in CRC tissue and paired normal tissue. ****, p < 0.0001. d and e Negative correlation of METTL14 and XIST expression in CRC tissue from our center (d) or from TCGA public dataset (e). R and p values were as indicated. f and g Cell count assay and transwell assay showed the effects of METTL14 and XIST on the proliferative and invasive ability of CRC cells. Inhibition of XIST suppressed the increased cell growth (f) and invasive ability (g) resulted from knockdown of METTL14. *, p < 0.05. **, p < 0.01. All data were representative of three independent experiments. Means ± SD were shown. Statistical analysis was conducted using Student’s t-test. Correlation of METTL14 and XIST was evaluated by Pearson’s correlation analysis