Fig. 6

Intercellular transfer of exosomal wtEGFR confers osimertinib resistance to mutEGFR H1975 xenograft in vivo. a Schematic diagram depicting the in vivo experimental procedure. b Line graph showing the change in body weight of mice during experiment (n = 7). c Comparison of the tumor volume of three indicated treatment groups at end point (n = 7). d Comparison of the tumor weight of three indicated treatment groups at end point (n = 7). e Tumor growth curve at the indicated days with different treatments in BALB/c-nu/nu mice (n = 7). f Macroscopic view of tumor harvested at termination of three indicated treatment groups (n = 7). g Western blot analysis of indicated proteins level in xenograft tumors from three indicated treatment groups. h Working model showing the induction of osimertinib resistance by intercellular delivery of wtEGFR protein in exosomes from osimertinib-insensitive wtEGFR-expressing NSCLC cells to osimertinib-sensitive mutEGFR NSCLC cells. All data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001