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Fig. 4 | Molecular Cancer

Fig. 4

From: Circular RNA circSDHC serves as a sponge for miR-127-3p to promote the proliferation and metastasis of renal cell carcinoma via the CDKN3/E2F1 axis

Fig. 4

miR-127-3p inhibits RCC progression through downregulation of CDKN3/E2F1 axis. a. The binding site between miR-127-3p (orange) and CDKN3 (blue) predicted by ENCORI. b. Luciferase reporter assay of 786-O with Luc-CDKN3-wt or Luc-CDKN3-mut co-transfected with mimics miR-127-3p or mimics NC. c. Western blot of CDKN3 levels after 786-O cells were treated with miR-127-3p inhibitor, mimics miR-127-3p or negative control. d and f. Cell migration and invasion abilities of 786-O and A498 transfected with CDKN3 siRNAs or control vector. e and g. Cell proliferation ability of 786-O and A498 transfected with CDKN3 siRNAs or control vector. h. GSEA analysis of E2F1 pathway in TCGA patients with high and low CDKN3 expression (NES, normal enrichment score; FDR, false discovery rate). i. Correlation analysis between CDKN3 and E2F1 from GEPIA website (TCGA dataset, n = 523). j and k. Western blot of CDKN3 and E2F1 levels after 786-O and A498 cells were transfected with CDKN3 siRNAs or control vector

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Molecular Cancer

ISSN: 1476-4598