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Fig. 2 | Molecular Cancer

Fig. 2

From: CircWAC induces chemotherapeutic resistance in triple-negative breast cancer by targeting miR-142, upregulating WWP1 and activating the PI3K/AKT pathway

Fig. 2

Characterization of circWAC as a circRNA in TNBC. a Information about the exonic structure of circWAC is illustrated as indicated. The specific primers used for validation of circWAC by Sanger sequencing. b qRT-PCR products generated with divergent primers showing circularization of circWAC. cDNA, complementary DNA. gDNA, genomic DNA. c qRT–PCR analysis of the expression of circWAC and WAC mRNA in MDA-MB-231 cells after treatment with RNase R. d qRT–PCR analysis of the expression of circWAC and WAC mRNA in MDA-MB-231 cells after treatment with actinomycin D at the indicated time points. e Nucleocytoplasmic fractionation experiment showing that circWAC was mainly distributed in the cytoplasm. f FISH analysis of circWAC. Nuclei were stained with DAPI. g The expression level of circWAC in a normal breast cell line (MCF-10A) and breast cancer cell lines was determined by qRT-PCR. The data are presented as the means±S.D. of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598