Fig. 3

MSH2 and circLIFR can improve CDDP chemosensitivity. a Gene set enrichment analysis (GSEA) of TCGA datasets showed that higher MSH2 expression was significantly associated with DNA repair and apoptosis in bladder cancer. b, c T24 cells were stably transfected with scramble, shMSH2#1, or shMSH2#2 vector. After T24 cells were treated for 36 h in the absence or presence of 5 μM CDDP, apoptosis was measured by Annexin-V plus PI staining and fluorescence-activated cell sorter (FACS) analysis. Bars show the percentages of cells that were early apoptotic (Annexin-V⁺/PI⁻) and late apoptotic/dead (Annexin-V⁺/PI⁺). Data were mean ± SD. ***P < 0.001, ****P < 0.0001 (Student’s t-test). d Determination of IC50 values for CDDP treatment 24 h in T24 cells which were stably transfected with scramble, shMSH2#1, shMSH2#2, mock, or MSH2 vector. e, f T24 cells were stably transfected with scramble, sh-circLIFR#2 vector. After T24 cells were treated for 36 h with or without 5 μM CDDP, apoptosis was measured by Annexin-V plus PI staining and FACS analysis. Data were mean ± SD. **P < 0.01, ****P < 0.0001 (Student’s t-test). g Determination of IC50 values for CDDP treatment 24 h in T24 cells which were stably transfected with scramble, sh-circLIFR#2, vector, or circLIFR. h Determination of IC50 values for CDDP treatment 24 h in T24 and T24-CDDP cells. i, j T24-CDDP cells were stably transfected with vector or circLIFR. After T24-CDDP cells were treated for 36 h in the absence or presence of 5 μM CDDP, apoptosis was measured by Annexin-V plus PI staining and FACS analysis. Data were mean ± SD. ns, not significant, **P < 0.01, ***P < 0.001 (Student’s t-test)