Fig. 7

Frequently mutated genes related to ENKTL identified by NGS. a The frequency of mutated genes is shown with the last row indicating the first gene with 1 recurrent mutation. Bar (left) represents the number of samples with mutations and bar (top) represents the number of mutations in each sample. b Percent of patients who had STAT5A/B mutations. c Locations of STAT5A/B mutations in ENKTL. d NK-92 cells were transduced with empty vector, STAT5A^WT, p.R131g, p.D475N, p.F706fs, p.V707E and p.S710F expression vectors. The PD-L1, p-STAT5, and total STAT5 protein levels in these cells were detected with Western blot. e NK-92 cells were transduced with empty vector, STAT5B^WT, p. R423Q, p.T628S, p.S434L expression vectors. The PD-L1, p-STAT5, and total STAT5 protein levels in these cells were detected with Western blot. f NK-92 cells which were transduced with empty vector, STAT5A^WT, p.R131G, p.D475N, p.F706fs, p.V707E, and p.S710F expression vectors were treated with GM-CSF (100 ng/ml) for 12 h. The PD-L1, p-STAT5, and total STAT5 protein levels in these cells were detected by Western blot. g STAT5A/B mutation rate of 144 melanoma patients reported by David Liu et al. [38]. h Scatter plots represent the CD274 mRNA of 144 melanoma patients (STAT5A mutant patients vs. STAT5A wildtype patients). I Scatter plots represents the CD274 mRNA of 144 melanoma patients (STAT5B mutant patients vs. STAT5B wildtype patients)