Fig. 1

circSPARC was upregulated in CRC. A. Heatmap of RNA-Seq analysis of top 10 differentially high expressed circRNAs generated from three paired of CRC and corresponding adjacent non-tumoral tissues. Red in the heatmap denotes upregulation, green denotes downregulation. B. The expression of the top 10 differentially high expressed circRNAs were verified in CRC and corresponding adjacent non-tumoral tissues by RT-qPCR. C. The intersection of RNA-seq results and three data from the GEO database. D. The co-localization of epithelial marker E-Cadherin and circSPARC was applied by FISH-IF staining assay in CRC and corresponding adjacent non-tumoral tissues (200X). The results showed that circSPARC expressed higher in tumor tissues. E. RT-qPCR was utilized to analyze the circSPARC expression in 84 pairs of CRC tissues and corresponding adjacent non-tumoral tissues. F. Expression of circSPARC was detected by RT-qPCR in 40 pairs of pre-surgery and post-surgery patients plasm. G. RT-qPCR was applied to analyze the circSPARC expression in 40 pairs of CRC patients and general people plasm. H. ROC analysis of the prognostic sensitivity and specificity for CRC patients and general people detected by the expression of circSPARC in plasm. I. Kaplan-Meier overall survival curves according to circSPARC expression in our cohort. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001