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Fig. 2 | Molecular Cancer

Fig. 2

From: The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway

Fig. 2

The characteristics of circSPARC. A. Schematic illustration indicating the generation of circSPARC from its host gene, and validation by Sanger sequencing. B. RT-qPCR for the abundance of circSPARC and its host gene mRNA in CRC cells treated with RNase R. C. PCR assay with divergent (◀▶) and convergent (▶◀) primers showing the amplification of circSPARC from cDNA or gDNA of CRC cell lines, while 18 s was used as a positive control. It can be observed that circSPARC was amplified in cDNA but not in gDNA while linear SPARC was amplified in both. D. Transcript half-life of circSPARC and its linear transcript in HCT116 cells treated with the transcription inhibitor actinomycin D. E. RT-qPCR indicated the circSPARC expressed higher in CRC cell lines (HCT116, DLD1, LoVo, SW480, SW620 and HT29) than in the normal colorectal epithelium cell line (FHC). F and G. FISH assay and nuclear-cytoplasm separation RT-qPCR data showed that circSPARC existed in both cytoplasm and nucleus, but primarily localized in the cytoplasm in CRC cells. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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Molecular Cancer

ISSN: 1476-4598