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Fig. 3 | Molecular Cancer

Fig. 3

From: The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway

Fig. 3

circSPARC regulated the proliferation and invasion of CRC cells and CTCF may function as the upstream of circSPARC. A and B. The transwell assay was performed for invasion and migration of CRC cells. C. The wound healing assay was performed for migration of CRC cells. D. The colony formation assay was performed for proliferation of CRC cells. E. The CCK-8 assay was performed for proliferation of CRC cells. F. TRcirc tool predicted that CTCF can function as the potential upstream of circSPARC. G. GEPIA database exhibited that CTCF is upregulated in CRC. H. GEPIA database showed that CTCF has a positive correlation with SPARC in CRC. I. RT-qPCR results showed that the level of SPARC pre-mRNA, circSPARC and SPARC mRNA were all decreased when CTCF was knockdown in HCT116 and DLD1 cells. J. P1-P9 showed the regions of SPARC promoter detected by the paired primers. ChIP assay revealed that CTCF mainly binds at P5, P6 and P7. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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Molecular Cancer

ISSN: 1476-4598