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Fig. 4 | Molecular Cancer

Fig. 4

From: The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway

Fig. 4

circSPARC regulated the JAK2/STAT3 signalling pathway. A and B. RNA-seq results showed that circSPARC had connection with JAK/STAT signalling pathway. C. RT-qPCR results showed that circSPARC had positive regulation on JAK2 but not STAT3. D. Western blot analysis showed that both silencing and overexpression circSPARC can regulate JAK2/STAT3 signalling pathway and its downstream genes expect STAT3. E. FISH-IF assay showed that JAK2 expressed higher in CRC tissues with high level of circSPARC. F and G. The transwell assay showed that the enhanced invasion and migration abilities were reversed by TG101348, an inhibitor of JAK2/STAT3 signalling. H. The wound healing assay showed that the enhanced migration abilities were reversed by TG101348, an inhibitor of JAK2/STAT3 signalling. I and J. The colony formation and CCK-8 assay showed that the enhanced proliferation abilities were reversed by TG101348. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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Molecular Cancer

ISSN: 1476-4598