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Fig. 5 | Molecular Cancer

Fig. 5

From: The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway

Fig. 5

miR-485-3p was directly bound to circSPARC and suppresses JAK2 activity. A and B. miR-485-3p, miR-646 and miR-663b were predicted that they can bind to circSPARC by two databases and were verified in CRC cells. C. RT-qPCR was utilized to analyse the miR-485-3p expression in 84 pairs of CRC tissues and corresponding adjacent non-tumoral tissues. D. The negative interaction within miR-485-3p and circSPARC. E. Luciferase reporter assay functionally verified the interaction within the circSPARC and miR-485-3p in 293 T cell. F and G. RT-PCR and western blot analysis detected the JAK2 level with transfecting miR-485-3p mimics or inhibitor in CRC cell lines. H. Luciferase reporter assay functionally verified the interaction within the JAK2 and miR-485-3p in 293 T cell. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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Molecular Cancer

ISSN: 1476-4598