Fig. 6

circSPARC regulates JAK2 expression and inhibits CRC cells activities by targeting miR-485-3p. A and B. The transwell assay demonstrated that cell migration and invasion abilities of CRC cells transfected with sh-circSPARC were counteracted when co-transfected with miR-485-3p inhibitor. C. The wound healing assay demonstrated that cell migration abilities of CRC cells transfected with sh-circSPARC were counteracted when co-transfected with miR-485-3p inhibitor. D and E. The colony formation and CCK-8 assay demonstrated that cell proliferation abilities of CRC cells transfected with sh-circSPARC were counteracted when co-transfected with miR-485-3p inhibitor. F. RT-qPCR results showed that the level of JAK2 transfected with circSPARC plasmid was reversed when co-transfected with miR-485-3p mimics in CRC cells. G. Western blot analysis showed that the level of JAK2 and its downstream genes transfected with sh-circSPARC was rescued when co-transfected with miR-485-3p inhibitor in CRC cells. H. Luciferase reporter assay functionally showed that overexpression of circSPARC increased the activity while co-transfection of the miR-485-3p mimic could eliminate this effect on the wild-type JAK2 sequence in 293 T cell. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001