Fig. 7

FIR reverses the oncogenic effects of circACTN4 in BC cells. a The ability of cell migration was detected by wound healing assay after transfection or co-transfection with indicated vectors or si-circ#2 (magnification, × 50, Scale bar, 200 um) . b The ability of cell proliferation was evaluated after transfection and co-transfection with circACTN4 plasmid, si-circACTN4, OE-FIR or sh-FIR vectors by EdU (magnification, × 100, Scale bar, 100 um) assay. c and d The invasion and migration abilities of BC cells transfected or co-transfected with circACTN4 plasmid, si-circACTN4, OE-FIR or sh-FIR vectors were measured by transwell invasion and transwell migration (magnification, × 100, Scale bar, 100 um), respectively. e and f The expression of MYC in BC cells transfected or co-transfected with indicated vectors and siRNA was detected by IF (magnification, × 200, Scale bar, 200 um). g The expression of MYC in breast cancer and paracancer tissues was displayed by IF (magnification, × 400, Scale bar, 50 um). h The expressions of MYC and its downstream proteins CDK4 and CCND2 were detected after transfection and co-transfection with indicated vectors and siRNA by western blot. GAPDH was used as the normalizing gene in the above experiments. The data are presented as the mean ± SD, *P < 0.05,**P < 0.01, ***P < 0.001