Fig. 3
From: A novel class of tsRNA signatures as biomarkers for diagnosis and prognosis of pancreatic cancer
The production mechanisms and biological functions of the two tsRNAs. A Representative images (left) and statistics analysis (right) of ANG staining from 60 PC tissues (PC) and 60 matched normal adjacent tissue (N). B TCGA dataset analysis of ANG in 179 pancreatic cancerous tissues (PC) and 171 normal pancreatic tissues (Normal). C For survival analyses, IHC intensities of ANG protein in PC tissues and paired normal adjacent tissues (n = 60) were collected and analyzed based on the ratio of IHC intensities (PC tissues/ paired normal adjacent tissues). The median of the data set was calculated as 1.11. And the median value 1.11 was set as cut-off value, > 1.11 as high expression and ≤ 1.11 as low expression. D PANC1 cells were transfected with control siRNA or ANG siRNA. Total RNA was extracted and subjected to qRT-PCR detection of tRF-Pro-AGG-004 and tRF-Leu-CAG-002. E-I Intravenous injection of ANG siRNA inhibited tumor growth and tsRNAs expression in vivo. E Flow chart of the experimental design. The PC orthotopic transplantation model was constructed using PAN02 cells in C57BL/6 J mice. Then the mice were randomly divided into two groups and intravenous delivered cholesterol-modified ANG siRNA (ANG-si) or control siRNA (si-CTL) every 3 days for 4 weeks (7 mice/group). F Tumor image at the day 28. G Quantitative analysis of tumor weights. H, I Quantitative analysis of two tsRNAs levels in tumor and serum in mice from two groups. J-O tRF-Pro-AGG-004 and tRF-Leu-CAG-002 function in pancreatic cancer. J Flow chart of the experimental design. PANC1 cells infected with control lentivirus (Normal), tRF-Pro-AGG-004 and tRF-Leu-CAG-002 overexpression lentivirus (tsRNAs) were implanted subcutaneously into nude mice (5 mice/group), and tumor growth was evaluated on day 30 after implantation. K Tumor images. L, M Quantitative analysis of tumor weights and tumor volumes. N, O Representative images and histogram statistics from EdU and invasion assays of PANC1 cells transfected with tsRNA mimics or inhibitors. *P < 0.05; **P < 0.01; ****P < 0.0001