Fig. 2

CircDIDO1 overexpression inhibits GC growth and metastasis in vitro and in vivo. a–e) Cell growth curve (a), colony formation (b), transwell migration (c), matrigel invasion assays (d) and flow cytometry analyses (e) for control and circDIDO1 overexpressing GC cells. Data are shown as means ± SD (n = 3, **P < 0.01). Scale bars = 100 μm. f) Balb/c nude mice were subcutaneously injected with control or circDIDO1 overexpressing HGC-27 cells. The size and weight of xenograft tumors were shown. Data are shown as means ± SD (n = 5 for each mice group, **P < 0.01). g) HE, Ki-67 and TUNEL staining of xenograft tumor tissues. Scale bars = 100 μm. h–i) Balb/c nude mice were intraperitoneally injected with control or circDIDO1 overexpressing HGC-27 cells. The number of peritoneal metastatic tumors and liver metastatic tumors was shown. Data are shown as means ± SD (n = 5 for each group, **P < 0.01)