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Fig. 2 | Molecular Cancer

Fig. 2

From: Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

Fig. 2

CircEYA3 facilitates the proliferation, migration, and invasion and inhibits the apoptosis of PDAC cells in vitro. A Three siRNAs targeting the circEYA3 junction site were constructed. B The expression of circEYA3 and EYA3 mRNA in PANC-1 cells was analysed by qRT-PCR after transfection with three siRNAs or the control siRNA (si-NC). C The levels of circEYA3 and EYA3 mRNA in MiaPaCa-2 cells were analysed by qRT-PCR after stable transfection with the circEYA3 overexpression vector (pLCDH-circEYA3) or the control vector (pLCDH-circ). D, E and F. Cell proliferation was assessed by CCK-8 (D), colony formation (E), and EdU incorporation assays (F). Scale bars, 100 μm. Downregulation of circEYA3 significantly inhibited the proliferation of PANC-1 cells, while ectopic upregulation of circEYA3 promoted the proliferation of MiaPaCa-2 cells. G. Apoptosis of PDAC cells was evaluated by Annexin V-PE/7-AAD staining when circEYA3 was downregulated and upregulated. H. Transwell assays were used to evaluate the migratory and invasive capabilities of PDAC cells when circEYA3 was downregulated or upregulated. Scale bars, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598