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Fig. 4 | Molecular Cancer

Fig. 4

From: Circular RNA CircEYA3 induces energy production to promote pancreatic ductal adenocarcinoma progression through the miR-1294/c-Myc axis

Fig. 4

miR-1294 inhibits the proliferation, migration, and invasion and promotes the apoptosis of PDAC cells in vitro. A and B. qRT-PCR was performed to evaluate the expression of miR-1294 in paired PDAC and matched adjacent normal tissues (n = 104) (A) and in PDAC cell lines and an HPDE cell line (B). C, D and E. Cell proliferation was assessed by CCK-8 (C), colony formation (D), and EdU incorporation assays (E). Scale bars, 100 μm. Upregulation of miR-1294 significantly inhibited the proliferation of PANC-1 cells, while ectopic downregulation of circEYA3 promoted the proliferation of MiaPaCa-2 cells. F. Apoptosis of PDAC cells was evaluated by Annexin V-PE/7-AAD staining when miR-1294 was upregulated or downregulated. H. A Transwell assay was performed to evaluate the migratory and invasive capabilities of PDAC cells when miR-1294 was upregulated or downregulated. Scale bars, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598